Prevention Clogging and Cleaning Rules of Glass Solvent Filter

In the operation of the glass solvent filter, there are some commonly occurred malfunctions such as clogging、low filtration, and so on. When using water or buffer salt as the mobile phase, you must change the mobile phase frequently. The water is best changed every day, at least once every two days. The buffered salt is best prepared for current use. If the mobile phase that is prone to deterioration is not replaced in time, it is easy to breed microorganisms, which will affect the stability of the baseline and even block the filter.

1. The mobile phase must be strictly filtered before entering the solvent bottle.
2. The aqueous mobile phase, especially the phosphate buffer system, must be prepared now, and must not be used if it has been stored for more than 2 days.
3. If it does not affect the experiment, 0.1-1 mM sodium azide can be added.
4. Add multi-functional bottle head assembly of the solvent bottle (more luxurious).
5. A brown solvent bottle must be used for the aqueous mobile phase or phosphate buffer system.

What if the glass solvent filter is really clogged, or if the sieve plate becomes discolored after a long time, and you want to clean it, what do you need to do?
1. Remove the solvent filter from the pipeline street.
2. Soak the filter in 35% concentrated nitric acid for about 1 hour.
3. Take it out and clean it with pure water, otherwise, it will damage the chromatographic column.
4. Put the filter back in place
Remember, the glass solvent filter cannot be cleaned with an ultrasonic cleaner, and the glass particles may shatter and cause blockage.

300ml Glass Solvent Filters Supplier vacuum filtration 300ml Glass Solvent Filters Packaging

Prevention of Clogging

  1. Use Prefilters:
    • Install prefilters or coarse filters upstream of the glass solvent filter to remove larger particles and debris before they reach the glass filter. This can extend the life of the glass filter.
  2. Proper Sample Preparation:
    • Ensure that the samples are properly prepared and free from particulate matter. Filtration is more effective when starting with a clean sample.
  3. Selection of Filter Pore Size:
    • Choose the appropriate pore size for the glass solvent filter based on the size of particles you need to remove. Using a filter with too small a pore size can lead to rapid clogging.
  4. Avoid High Particulate Loads:
    • Be cautious when filtering samples with high particulate loads. If necessary, use a series of filters with progressively smaller pore sizes to reduce the load on each filter.
  5. Use Compatible Solvents:
    • Ensure that the solvents used are compatible with the glass filter material. Some solvents may degrade or swell certain filter materials, leading to clogging or damage.
  6. Regular Monitoring:
    • Monitor the pressure across the glass filter during filtration. A sudden increase in pressure may indicate clogging, and filtration should be paused to inspect and clean the filter.

Tips to Tell Clogged of Glass Solvent Filter

To determine whether the solvent filter is blocked, you can remove the solvent inlet line from the instrument, because the line is full of the mobile phase. If there is no blockage, the mobile phase will flow out freely from the line. Otherwise, no mobile phase will flow out or only a very small amount of outflow.
The filter head of the glass material is clogged. Ultrasonic cleaning is not recommended, because ultrasound can easily damage the filter plate of the glass filter. It is only recommended to soak in dilute nitric acid (about 35%). After soaking for about an hour, wash the filter head with water; and the stainless steel filter head can be ultrasonically cleaned.

Glass solvent filters have a service life, which is a great factor but many users don’t know. When the glass solvent filter is unqualified and contaminated, samples will be affected to great extent, thus microorganisms will exist in the solvent filter, and directly reduce the performance and cause blockage in the operation process, and the service life of the solvent filter will shorten.

Cleaning Rules for Glass Solvent Filter

  1. Frequency of Cleaning:
    • Establish a regular cleaning schedule based on the nature of the samples being filtered and the volume of filtration. For continuous or high-volume use, more frequent cleaning may be required.
  2. Immediate Cleaning After Use:
    • Clean the glass solvent filter immediately after each use. This prevents the accumulation of dried or hardened residues that can be more challenging to remove.
  3. Use Compatible Cleaning Agents:
    • Select cleaning agents that are compatible with the glass filter material and the substances being filtered. Common cleaning agents include isopropanol or methanol.
  4. Backflushing:
    • Consider backflushing the glass solvent filter periodically. This involves reversing the flow direction to dislodge and remove particles trapped on the filter surface.
  5. Ultrasonic Cleaning:
    • If applicable, use ultrasonic cleaning to remove stubborn residues from the glass filter. Ultrasonic cleaning can be particularly effective for intricate or hard-to-reach areas.
  6. Visual Inspection:
    • Regularly inspect the glass filter for any visible signs of damage, discoloration, or degradation. Replace the filter if it shows signs of wear.
  7. Drying:
    • Allow the glass solvent filter to dry thoroughly after cleaning to prevent the growth of microorganisms and the formation of deposits.
  8. Documentation:
    • Keep a record of cleaning procedures, including the cleaning agents used, frequency of cleaning, and any issues observed. This documentation can help in troubleshooting and maintaining consistent performance.
  9. Replace if Necessary:
    • If cleaning no longer restores the glass solvent filter to its optimal condition, or if it shows signs of damage, consider replacing it with a new filter.

It is recommended to clean or replace the new filter regularly every 3 months. Besides, a small glass device is also named a glass solvent filter. This glass solvent filter is used to filter the organic or aqueous mobile phase before the mobile phase is put into the solvent bottle, and a vacuum pump is required for use. If the solvent filter is clogged, or the sieve plate becomes discolored after a long time, what should I do to clean it?
1. Remove the solvent filter from the street of the pipeline;
2. Take it out and clean it with pure water, otherwise, it will damage the chromatographic column;
3. Put the filter back in place.

The glass solvent filter should not clean with the ultrasonic cleaner to avoid the glass particles may shatter and cause blockage.
There are some ways to decide whether the glass solvent filter is clogged in the operation process. To determine whether the solvent filter is blocked, you can remove the solvent inlet line from the instrument, because the line is full of the mobile phase. If there is no blockage, the mobile phase will flow out freely from the line. On the contrary, no mobile phase will flow out or only a very small amount of outflow.
In summary, prevention is the key point. Cleaning is only a last resort. If it is not clean, you need to replace it with a new one.

Wrong Operations in Cleaning Glass Solvent Filter

Some operators open the solvent bottle directly, or simply cover it, and some customers use methanol and acetonitrile for mobile phase preparation that is themselves substandard. These are very easy to cause solvent pollution, not to mention harmful to the experiment.
The direct consequence of this is that after a long time of accumulation, the solvent filter will become clogged, the pressure will rise, and the instrument will malfunction. The above operation should be avoided to prolong the service life of the glass solvent filter.