A Brief Introduction of Vacuum Filtration

Vacuum filtration is a physical term, also known as decompression filtration and vacuum filtration, which has double meanings. In physical terms, vacuum filtration refers to the method of reducing the pressure in the vacuum filtration bottle by using the vacuum pump to achieve the purpose of solid-liquid separation.

Required device of vacuum filtration

Buchner funnel, vacuum bottle, hose, vacuum pump( Standard Diaphragm Vacuum Pumps and Anti-Corrosion Diaphragm Vacuum Pump ), filter paper.
Hawach provided Glass Single Vacuum Filtration Assembly Glass Triple-Branches Lab Vacuum Filtration System, and Glass 6-Branch Vacuum Filtration Kit for your choice.

Process of vacuum filtration

First of all, the instrument shall be installed to check whether the connection between the Buchner funnel and the vacuum filter bottle is tight and whether the connection of the vacuum pump is leaking; the vacuum filter bottle is equipped with a single hole plug, and the Buchner funnel is installed in the plug hole. The inclined surface at the lower end of the funnel tube faces the air extraction nozzle. But it is not reliable to be too close, so as to avoid the filtrate being pumped away from the vacuum nozzle.

Trim the filter paper to make it slightly smaller than the cloth funnel, but cover all the holes, and drip distilled water to make the connection between the filter paper and the funnel close; add a small amount of water or solvent to the filter paper, turn on the tap gently, and suck part of the air in the vacuum bottle to make the filter paper close to the bottom of the funnel, so as to avoid solid entering the filtrate from the edge of the filter paper during the filtration process. The third step is to turn on the air pump switch and start vacuum filtration. In the process of vacuum filtration, when the solid layer in the funnel cracks, the glass plug, and other things shall be used to compress it and block the cracks.

Glass Three-Branches Vacuum Filtrations
SLVPGM100A-T-Anti-Corrosion-Diaphragm-Vaccum-Pumps

If it is not pressed tightly, it will also reduce the efficiency of vacuum and filtration; if the solid needs to be washed, a small amount of solvent can be sprinkled on the solid, stand for a while, and then pump it dry. When taking out the solid from the funnel, remove the funnel from the vacuum filter bottle, hold the funnel tube with the left hand, turn it upside down, and “tap” the left hand with the right hand, so that the solid, together with the filter paper, falls onto the clean paper piece or surface dish. Remove the filter paper and dry the solid. The solution should be poured out from the top of the vacuum flask.

Matters needing attention of vacuum filtration

1. When the vacuum filtration is stopped, first unscrew the cock on the safety bottle to restore normal pressure and then close the air extraction pump
2. When the filtered solution has strong acidity, strong alkalinity or strong oxidation, use glass fiber instead of filter paper or glass sand funnel instead of Brinell funnel
3. It is not suitable to filter colloidal precipitation or precipitation with too small particles.

Hawach vacuum filtration is made of transparent polymer material polystyrene (GPPS), which uses a vacuum pump to provide pressure difference for large-scale filtration of tissue culture fluid and other laboratory fluid solutions. The sample throughput can reach several liters, and the filtered sample It can be stored directly in a sterile collection bottle.

The vacuum filtration uses a vacuum pump to provide a pressure differential for bulk filtration of tissue culture fluids and other laboratory fluid solutions. Samples can be processed up to several liters and the filtered samples can be stored directly in sterile collection bottles. Hawach offers a wide range of vacuum filtration units in three main forms: full filtration/storage unit, top filter and receiving a bottle.

The vacuum filtration is a disposable filter consumable that replaces the solvent filter. It is aseptically packaged separately, and the filter membrane of the corresponding specification is welded inside. When it is used up, the trouble of cleaning and disinfection is saved, and a lot of labor is saved. The filter is made of PS material and is used with a diaphragm vacuum pump. It can be used to filter large-volume samples. The filtered sample can be directly stored in the collection box and screwed onto the lid.